Y the volume ( ml).For each and every mucin grade the imply density with regular deviation was determined.Sialic acidThe sialic acid content of your solubilised mucin samples was determined using the BIO VISION Sialic Acid (NANA) ColorometericFluorometeric Assay Kit (Milpatas, CA, USA).Solubilised mucin samples were diluted and tested as well as prepared sialic acid common curve as Pleuromutilin Purity & Documentation suggested by the supplier.Absorbance (OD) was measured at m.Imply values were generated for each and every grade of mucin working with common methods, as just before.Kinematic viscosityThe kinematic viscosity of each solubilised mucin sample was determined applying methods by Fries et al. Briefly the time in seconds and the height the liquid rises in an inclined capillary tube (.mm in diameter) touching the surface of the liquid was measured at ambient room temperature of o C.Imply values with typical deviation was determined for each grade of mucin.GlucoseGlucose in the solubilised mucin samples are measured quantitatively utilizing the phenolsulphuric acid system as carried out by Masuko et al. Briefly, of concentrated sulphuric acid and of phenol in water had been added in rapid succession to of mucin answer (dilution , ,,) within a microwell plate.Just after incubation for minutes at o C in static water bath by floating the microplate cautiously.The plate was then cooled to space temperature for min in a water bath and wiped dry to measure absorbance at m by a microplate reader.Typical curves have been ready for glucose (, , , , ml) within a related style.Imply values have been generated, utilizing procedures as just before.Percentage Water content (fresh mucin samples)A identified weight (..g) of every single mucin sample was placed within a Petri dish and incubator dried ( o C) over hours.The residual weight with the mucin was measured and percentage hydration was calculated making use of the below formula.N [ (X Y) X] Exactly where N Percentage hydration; X Weight of mucin just before drying (g); Y Weight of mucin after drying (g) Imply percentage of water for every single grade of mucin was determined as before.LipidThe mean lipid concentration inside the solubilised mucin samples have been determined employing the Cholesterol Quantification kit (SIGMA ALDRICH catalogue No.MAK) inside a micro well plate.Briefly, utilizing cholesterol requirements, a standard curve was generated for the detection of , .and .gwell and solubilised mucin samples had been diluted to read inside this level.Colorimetric readings, Absorbance (OD) have been carried out atwww.medsci.orgDensity (fresh mucin samples)The density of fresh samples were determined applying PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21600948 the water displacement process, Briefly .g of mucin sample was weighed and immersed in distilled water at area temperature, the density was determined using standard formula, Mass Volume.Int.J.Med.Sci Vol.m following protocol as advised by the manufacturer.Mean values had been calculated for every mucin grade, as prior to.Search Pty Ltd.ResultsAll information have already been plotted against hardness index (HI) a measurement technique that has been developed by our group to classify the hardness of PMP mucin into 3 categories according to the location, a unit weight (g) of mucin fully hydrated (soaked for mins in distilled water) occupies when placed on a gridded glass slab (marked in mm).The HI index of . soft mucin; .. semi tough mucin and . hard mucin (publication in press)Thiol (SS and SH)To measure total sulfhydryl (SH) content, .g of mucin was very carefully weighed into a ml centrifuge tube and lowered with .M NaBH in M UreamM Na EDTA.M NaHPO NaHPO, pH.
