Lysis and image) were treated as hepatorenal syndrome with terlipressin (0.5? mg iv every 4? hrs) plus albumin for at least 3 days. Others were treated as intrinsic azotemia as described above [4].Statistical analysisDescriptive statistics were expressed as mean and standard deviation values unless otherwise stated. In the primary analysis, we compared the number of hospital survivors with the number of nonsurvivors. Normal distribution of all the variables was analyzed using the Kolmogorov mirnov test. Student’s t-test was used to compare the mean values of continuous variables and normally distributed data; in the case of the other data, the Mann hitney U test 12926553 was used. Categorical data were analyzed using the x2 test. The chi-square test for trends were used to assess categorical data associated with MBRS scores. Correlation of paired-group variables were assessed using linear regression and Madrasin site Pearson analysis. We assessed the risk factors for in-hospital mortality by using univariate analysis, and the variables that were found to be statistically significant (p,0.05) in the univariate analysis were included in the multivariate analysis. A multiple logistic regressionNew Score in Cirrhosis with AKITable 2. Causes of cirrhosis, reasons for ICU admission and presumptive causes of AKI.All patients ( )Survivors ( )Non-survivors ( )pCauses of cirrhosis1948-33-0 supplier Alcoholic Hepatitis B Hepatitis C Alcoholic+Hepatitis B Alcoholic+Hepatitis C Hepatitis B+Hepatitis C Alcoholic+Hepatitis B+Hepatitis C Other causesa33 (17) 60 (32) 39 (20) 14 (7) 3 (2) 5 (3) 1 (1) 35 (17)15 (29) 6 (12) 11 (22) 8 (16) 
1 (2) 1 (2) 0 (0) 9 (18)18 (13) 54 (39) 28 (20) 6 (4) 2 (1) 4 (3) 1 (1) 26 (19)0.005 ,0.001 NS (0.716) 0.006 NS (0.771) NS (0.755) NS (1.000) NS (0.868)Primary ICU admissionSevere UGI bleeding Severe sepsis Hepatic encephalopathy Respiratory failure AKI require renal replacement Othersb 46 (24) 34 (18) 25 (13) 10 (5) 11 (6) 64 (35) 18 (35) 5 (10) 11 (22) 3 (6) 2 (4) 12 (24) 28 (20) 29 (21) 14 (10) 7 (5) 9 (6) 52 (37) NS (0.031) NS (0.078) 0.038 NS 23727046 (0.817) NS (0.504) NS (0.073)Presumptive etiology of AKIPre-renal failure Infection-induced AKI Parenchymal renal diseases Acute tubular necrosis Nephrotoxic acute renal failure HRS type I/type II/total Othersc 31 (16) 51 (27) 11 (6) 17 (9) 9 (5) 10/17/27 (14) 44 (23) 13 (25) 5 (10) 5 (10) 3 (6) 6 (12) 1/2/3 (6) 16 (31) 18 (13) 46 (33) 6 (4) 14 (10) 3 (2) 9/15/24 (17) 28 (20) 0.038 0.001 NS (0.151) NS (0.370) 0.006 0.046 NS (0.104)Abbreviation: UGI, upper gastrointestinal; AKI, acute kidney injury; NS, not significant; ICU, intensive care unit; HRS, hepatorenal syndrome. Primary biliary cirrhosis, autoimmune hepatitis, and other unknown causes. Pancreatitis, hepatoma rupture, unknown cause, or multifactor related. c Mixed type, unknown cause, or multifactor related. doi:10.1371/journal.pone.0051094.ta bmodel and forward elimination of data were used to analyze these variables. Calibration was assessed using the Hosmer emeshow goodness-of-fit test to compare the number of observed deaths with the number of predicted deaths in the risk groups for the entire range of death probabilities. Discrimination was calculated using the AUROC values. The AUROC values were compared using a nonparametric approach. The AUROC analysis was also utilized to calculate the cut-off values, sensitivity, specificity, and overall correctness. Finally, cut-off points were calculated by calculating the best Youden index (sensitivity+specificity21.Lysis and image) were treated as hepatorenal syndrome with terlipressin (0.5? mg iv every 4? hrs) plus albumin for at least 3 days. Others were treated as intrinsic azotemia as described above [4].Statistical analysisDescriptive statistics were expressed as mean and standard deviation values unless otherwise stated. In the primary analysis, we compared the number of hospital survivors with the number of nonsurvivors. Normal distribution of all the variables was analyzed using the Kolmogorov mirnov test. Student’s t-test was used to compare the mean values of continuous variables and normally distributed data; in the case of the other data, the Mann hitney U test 12926553 was used. Categorical data were analyzed using the x2 test. The chi-square test for trends were used to assess 
categorical data associated with MBRS scores. Correlation of paired-group variables were assessed using linear regression and Pearson analysis. We assessed the risk factors for in-hospital mortality by using univariate analysis, and the variables that were found to be statistically significant (p,0.05) in the univariate analysis were included in the multivariate analysis. A multiple logistic regressionNew Score in Cirrhosis with AKITable 2. Causes of cirrhosis, reasons for ICU admission and presumptive causes of AKI.All patients ( )Survivors ( )Non-survivors ( )pCauses of cirrhosisAlcoholic Hepatitis B Hepatitis C Alcoholic+Hepatitis B Alcoholic+Hepatitis C Hepatitis B+Hepatitis C Alcoholic+Hepatitis B+Hepatitis C Other causesa33 (17) 60 (32) 39 (20) 14 (7) 3 (2) 5 (3) 1 (1) 35 (17)15 (29) 6 (12) 11 (22) 8 (16) 1 (2) 1 (2) 0 (0) 9 (18)18 (13) 54 (39) 28 (20) 6 (4) 2 (1) 4 (3) 1 (1) 26 (19)0.005 ,0.001 NS (0.716) 0.006 NS (0.771) NS (0.755) NS (1.000) NS (0.868)Primary ICU admissionSevere UGI bleeding Severe sepsis Hepatic encephalopathy Respiratory failure AKI require renal replacement Othersb 46 (24) 34 (18) 25 (13) 10 (5) 11 (6) 64 (35) 18 (35) 5 (10) 11 (22) 3 (6) 2 (4) 12 (24) 28 (20) 29 (21) 14 (10) 7 (5) 9 (6) 52 (37) NS (0.031) NS (0.078) 0.038 NS 23727046 (0.817) NS (0.504) NS (0.073)Presumptive etiology of AKIPre-renal failure Infection-induced AKI Parenchymal renal diseases Acute tubular necrosis Nephrotoxic acute renal failure HRS type I/type II/total Othersc 31 (16) 51 (27) 11 (6) 17 (9) 9 (5) 10/17/27 (14) 44 (23) 13 (25) 5 (10) 5 (10) 3 (6) 6 (12) 1/2/3 (6) 16 (31) 18 (13) 46 (33) 6 (4) 14 (10) 3 (2) 9/15/24 (17) 28 (20) 0.038 0.001 NS (0.151) NS (0.370) 0.006 0.046 NS (0.104)Abbreviation: UGI, upper gastrointestinal; AKI, acute kidney injury; NS, not significant; ICU, intensive care unit; HRS, hepatorenal syndrome. Primary biliary cirrhosis, autoimmune hepatitis, and other unknown causes. Pancreatitis, hepatoma rupture, unknown cause, or multifactor related. c Mixed type, unknown cause, or multifactor related. doi:10.1371/journal.pone.0051094.ta bmodel and forward elimination of data were used to analyze these variables. Calibration was assessed using the Hosmer emeshow goodness-of-fit test to compare the number of observed deaths with the number of predicted deaths in the risk groups for the entire range of death probabilities. Discrimination was calculated using the AUROC values. The AUROC values were compared using a nonparametric approach. The AUROC analysis was also utilized to calculate the cut-off values, sensitivity, specificity, and overall correctness. Finally, cut-off points were calculated by calculating the best Youden index (sensitivity+specificity21.
