Ience and Biotechnology (2016) 7:Table three Reactive oxygen species (ROS) production by bovine
Ience and Biotechnology (2016) 7:Table three Reactive oxygen species (ROS) production by bovine spermatozoa [RLU/s/106 sperm] impacted by 4 doses of lycopene (LYC), untreated vs. treated with ferrous ascorbate (FeAA)Fractions Fractions untreated with FeAA Ctrl 1 (SC Ctrl) Time 0 h 1.63 Prostatic acid phosphatase/ACPP Protein custom synthesis sirtuininhibitor0.32 Time two h 4.49 sirtuininhibitor0.42 Time six h ten.92 sirtuininhibitor0.62 six.55 sirtuininhibitor0.211 7.01 sirtuininhibitor0.541 eight.66 sirtuininhibitor0.52 9.15 sirtuininhibitor0.36 23.55 sirtuininhibitor1.511 12.33 sirtuininhibitor1.092 14.77 sirtuininhibitor0.802 17.00 sirtuininhibitor1.212, 1 20.25 sirtuininhibitor1.561 2.49 sirtuininhibitor0.72 2.58 sirtuininhibitor0.50 3.66 sirtuininhibitor0.19 four.10 sirtuininhibitor0.25 9.75 sirtuininhibitor0.851 five.28 sirtuininhibitor0.222 5.79 sirtuininhibitor0.452 7.90 sirtuininhibitor0.481 eight.11 sirtuininhibitor0.651 1.01 sirtuininhibitor0.22 1.21 sirtuininhibitor0.30 1.30 sirtuininhibitor0.16 1.57 sirtuininhibitor0.30 4.22 sirtuininhibitor0.701 two.00 sirtuininhibitor0.492 two.12 sirtuininhibitor0.412 two.55 sirtuininhibitor0.39 three.16 sirtuininhibitor0.581 two mmol/L LYC 1 mmol/L LYC 0.5 mmol/L LYC 0.25 mmol/L LYC Fractions treated with FeAA Ctrl 2 (FeAA Ctrl) 2 mmol/L LYC 1 mmol/L LYC 0.5 mmol/L LYC 0.25 mmol/L LYCMean sirtuininhibitorStandard Error P sirtuininhibitor 0.05; P sirtuininhibitor 0.01; P sirtuininhibitor 0.001 1 sirtuininhibitorvs. Control 1 (SC Manage) two sirtuininhibitorvs. Handle 2 (FeAA Control)Page 7 ofTvrdsirtuininhibitoret al. Journal of Animal Science and Biotechnology (2016) 7:Web page eight ofFig. 2 Intracellular superoxide production of bovine spermatozoa affected by four doses of lycopene (LYC), untreated or co-treated with ferrous ascorbate (FeAA). Each bar represents imply ( EM) optical density because the percentage in the Control 1 (SC control), which was set to 100 plus the data are expressed as a with the Manage 1 worth. The data have been obtained from 5 independent experiments. The amount of significance was set at 0.05, and suggests P sirtuininhibitor 0.001, means P sirtuininhibitor 0.01, suggests P sirtuininhibitor 0.05. 1 sirtuininhibitorvs. Manage 1, two sirtuininhibitorvs. CD162/PSGL-1 Protein site ControlAssessment on the antioxidant profile revealed that FeAA treatment led to a significant lower of SOD, CAT and GPx activities (P sirtuininhibitor 0.01 with respect to SOD; P sirtuininhibitor 0.001 in relation with CAT and GPx) also as a decline of the GSH concentration (P sirtuininhibitor 0.05; Table four).Inversely, a significant boost of the MDA concentration (P sirtuininhibitor 0.001) was detected following FeAA treatment (Table four). Evaluating the influence of LYC on the intracellular antioxidant molecules below physiological situations it was revealed that 1 and 2 mmol/L LYC have been able toTvrdsirtuininhibitoret al. Journal of Animal Science and Biotechnology (2016) 7:Table four Markers of oxidative balance in bovine spermatozoa impacted by four doses of lycopene (LYC), untreated vs. treated with ferrous ascorbate (FeAA)Fractions Fractions untreated with FeAA Ctrl 1 (SC Ctrl) SOD, U/mg prot 0.43 sirtuininhibitor0.06 CAT, U/mg prot 9.14 sirtuininhibitor0.86 GPx, U/mg prot 0.088 sirtuininhibitor0.016 GSH, mg/g prot 8.10 sirtuininhibitor1.02 MDA, mol/g prot four.27 sirtuininhibitor0.24 3.09 sirtuininhibitor0.26 3.32 sirtuininhibitor0.33 three.33 sirtuininhibitor0.38 3.65 sirtuininhibitor0.38 ten.25 sirtuininhibitor0.751 four.21 sirtuininhibitor0.252 four.35 sirtuininhibitor0.242 four.52 sirtuininhibitor0.142 four.73 sirtuininhibitor0.
