S calculated and expressed on dry weight basis.Determination of cyanide
S calculated and expressed on dry weight basis.Determination of cyanide in brebra seed flourDetermination of ester valueThe content of cyanide in brebra seed flour was determined by the volume of HCN released on hydrolysis. Brebra seed flour extract was obtained by homogenizing 30 gm of flour in 259 ml of 0.1 M orthophosphoric acid for five min. The homogenate was centrifuged at two,500 rpm for 20 min and clear supernatant was taken. An aliquot on the supernatant was employed for determination of hydrogen cyanide using an auto analyzer Technicon AAII, in line with the method of Rao and Hahan (1984).Chemical characterization of brebra (Meillettia ferrugeniea) oilThe ester value can be a measure from the level of ester present in the offered oil. It is actually expressed within the identical terms as saponification value plus the acid worth. It was determined by subtracting the acid value in the saponification value (Ester value = Saponification value Acid value).Physical traits of oilDetermination of physical qualities such as moisture, specific gravity and density, Kinematic viscosity, refractive index and pH value had been carried out in accordance with the solutions of ASTM (2002)peting interests The authors declare that they have no competing interests. Authors’ contributions B-A Create and edits the whole manuscript and B-A supervised the general activities and reviewed the documents. B-A also involved in statistical analysis and preparation of this manuscript. Both authors have study and authorized the final manuscript. Authors’ information and facts Bothe B-A and A-G are KDM4 MedChemExpress Associate Professor and many of the time we’re engaged in analysis and management of diverse analysis projects. Acknowledgement We are thankful towards the University of Gondar for monetary help (UoG Budgetno.6215) for the thriving completion of this investigation operate. We would like to express our appreciation towards the Department of Biotechnology for the facilities provided for the duration of this investigation. Author information 1 Department of Biotechnology, Organic and Computational Sciences Faculty, University of Gondar, P.O. Box 196, Gondar, Ethiopia. 2Biotechnology Institute, Collage of Organic Sciences, Addis Ababa University, Addis Ababa, Ethiopia. Received: 3 April 2014 Accepted: 12 Might 2014 Published: 13 June 2014 References Abdullahi SA (1999) Nutrient content material of Citharinus citharus and C. latus. Zuma J Pure App Sci two:668 Achinewhu SC (1990) Composition and meals possible of melon seed (C. vulgaris). Nig Meals J 8:13033 Akinhanmi TF, Atasie VN, Akintokun PO (2008) Chemical Composition and Physicochemical Properties Of Cashew nut (Anacardium occidentale) Oil and Cashew nut Shell Liquid. J Agri Food Caspase 11 Storage & Stability Enviro Sci two(1) ISSN 1934-7235 Akintayo ET, Bayer E (2002) Characterization and a few probable utilizes of Plukenetia conophora and Adenopus breviflorus seeds and seed oils. Biores Tech 85:957 Akinyede AI, Amoo IA, Eleyinmi AF (2005) Chemical and functional properties of complete fat and defatted Dioclea reflexa seed flours. J Food Agri Envir three(two):11215 Akpan UG, Jimol A, Mohammed (2007) Extraction, characterization and modification of castor seed oil. Leonardo J Sci Situation 8:432, ISSSN 15832333 Alcantara R, Amores J, Canoira L, Fidalgo E, Franco MJ, Navarro A (2000) Catalytic production of biodiesel from soy-bean oil, used frying oil and tallow. Biomed and Bioen 18:51527 Aletor VA, Aladetimi OO (1989) Compositional evaluation of some cowpea varieties and a few under-utilized edible legumes in Nigeria. Die Nahrung 33(ten):999007.
