Tionally made use of as a diuretic but scientifically not evaluated as a diuretic agent. The key aim on the present study was to evaluate diuretic activity of roots of C. pareira in hydrated (Modified Lipschitz test ) albino rats.Plant DescriptionThe Cissampelos pareira [3], an extensively spreading, glabrous to soft pubescent, perennial climbing shrub found all over India and is frequently generally known as Padha along with other CDK7 Inhibitor Source synonyms are Padvel, Padvali, Aaknadi, Venievel, Poda and Patha belongs to the family of Menispermaceae [3]. In Ayurvedic system of medicine, the leaves and roots are used in the CYP26 Inhibitor Formulation remedy of indolent ulcers (Kirtikar and Basu,) and diarrhea (Amresh et al.,). The plant is used in the therapy of urinary tract infections since it is regarded as as antiseptic (Dandiya and Chopra,). Juice of C. pareira is given in migraine and the plant has a lengthy history of use for inflammation of muscles, snakebite, rheumatism, diarrhoea, dysentery and menstrual troubles. C. pariera is extensively employed in herbal medicine these days as a diuretic, tonic as well as to minimize fever and to relieve pain. It is usually employed for menstrual cramps, dysmenorrhoea, excessive bleeding and uterine hemorrhages, fibroid tumors, pre and postJournal of Clinical and Diagnostic Analysis. 2014 Might, Vol-8(5): HC01-HCMETHODOLOGYCollection of your PlantThe roots of C.pareira have been obtained from the forest of Tirupati, AP and had been identified and authenticated by Dr. Pramod Kumar, Pharmacognocist V.L. College of Pharmacy, Raichur, Karnataka, India.Preparation of ExtractRoots have been thoroughly washed beneath fresh tap water and shade dried and powdered by using a mechanical grinder. The preparationSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratsjcdr.netof alcoholic extract of roots of C.pareira was performed by using soxhletation within the Department of Pharmacology, V.L.College of Pharmacy, Raichur. About 200 g of root powder was taken into the soxhlet apparatus and extracted using ethanol (95 ). The extraction method was carried out for 18 – 20 h till the appearance of colourless solvent inside the side tube. The extract collected was dried by evaporating the solvents on a water bath maintained at 500C and percentage yield of alcoholic extract was recorded with respect towards the total quantity of powder applied for the extraction. Phytochemical evaluation for the extract was performed working with common procedures.kept at 21 ?.five . The total volume of urine collected for 5 hr was measured at the finish. For the duration of this period no meals and water was produced out there to animals. A variety of parameters like total urine volume and concentration of sodium, potassium and chloride inside the urine had been measured and estimated respectively.Estimation of Urinary ElectrolytesUrine electrolytes (sodium, potassium and chloride) had been determined by Ion Selective Electrode process as described by the user instruction manual of the biochemical kits (Roche, Roche Diagnostics Pvt. Ltd, Gurgaon, Haryana.)Experimental AnimalsAlbino rats weighing involving 140-200 g of either sex had been employed within the study and had been obtained in the Central Animal House, V.L.College of Pharmacy, Raichur, Karnataka, India. The experimental protocol was approved by the Institutional Animal Ethical Committee and these animals were applied to evaluate the diuretic activity of alcoholic extract of roots of Cissampelos pareira. The animals had been maintained under regular husbandry circumstances for an acc.
