Ract. For the inhibitory activity analysis, C2 showed 13.2 and 55.eight of residual activity for MAO-B and MAO-A, respectively, at two /mL; nevertheless, no substantial inhibitory activity was observed for AChE, BChE, or BACE-1 (Table 3). C1 showed pretty weak inhibitory activities for the enzymes. C1 and C2 showed quite weak antioxidant activity (Table three). For the reason that Compound C1 showed no substantial inhibitory activities and had an amount limitation, only compound C2 was further studied.J. Fungi 2021, 7, x FOR PEER REVIEW7 ofJ. Fungi 2021, 7,6 of100Residual activity ( )80 70 60 50 40 30 20 10 0 Extract 1 two 3 4 5 six 7Figure 1. Residual activities on the ELF13 extract and eight fractions from primary PTLC. The comFigure 1. Residual activities in the ELF13 extract and eight fractions from key PTLC. The compound was separated the initial solvent (ethyl acetate:mGluR1 manufacturer toluene = 1:9, v/v). The activityThe activity with the pound was separated with together with the initial solvent (ethyl acetate:toluene = 1:9, v/v). on the compound measured at 20 20 /mL. compound was was measured atg/mL.Table three. Inhibitory activities for the enzymes of two isolated compounds. Table three. Inhibitory activities for the enzymes of two isolated compounds.ELF13 C1 C1 CELFC2 The compounds have been separated using the second solvent (chloroform:toluene = 1:9, v/v). Outcomes are expressed as imply The compounds have been from 4-1BB Inhibitor Storage & Stability duplicate experiments. a Residual activity at ten g/mL. b Benefits are expressed g/mL. and standard deviationseparated with the second solvent (chloroform:toluene =:9, v/v).Concentration at one hundred s imply and standarddeviation from duplicate experiments. a Residual activity at ten /mL. b Concentration at one hundred /mL.Residual Activity at two /mL ( ) Residual Activity at two /mL ( ) MAO-A MAO-B AChE BChE BACE-1 a MAO-A MAO-B AChE BChE 57.1 2.72 89.3 three.68 81.0 2.01 80.6 1.31 93.4 1.02 57.1 two.72 89.three three.68 81.0 two.01 80.6 1.31 55.8 0.91 13.2 0.48 73.0 1.13 83.eight five.89 98.six 2.04 55.eight 0.91 13.two 0.48 73.0 1.13 83.eight five.b Inhibition Inhibition b DPPH BACE-1 a DPPH 9.58 0.29 93.four 1.02 9.58 0.29 25.11 two.12 98.6 2.04 25.11 two.three.3. Molecular Structure Evaluation of C2 3.three. Molecular Structure Analysis of C2 3 aromatic protons [H-6 (H 6.43), H-7 (H The 1H NMR spectrum of C2 revealed The 1 six.46)], one particular methylene proton [H-3 (H two.73)], one protons proton [H-2 ( 7.38), H-8 (H H NMR spectrum of C2 revealed 3 aromatic methine [H-6 (H 6.43),HH-7 (H 7.38), H-8 methyl doublet proton [H-2 (H 1.49)] (Figure S6). The 13 methine proton [H-2 (H four.55)], and one(H 6.46)], one methylene proton [H-3 (H 2.73)], oneC NMR and HMBC four.55)], and one particular displayed one carbonyl carbon [C-4 (C 199.6)], S6). The 13 C NMR and spectroscopic data methyl doublet proton [H-2 (H 1.49)] (Figure a single oxygenated carbon HMBC spectroscopic information displayed one [C-3 (C carbon [C-4 (C 199.6)], one particular oxygenated [C-2 (C 75.three)], 1 methylene carbon carbonyl42.8)], three quaternary carbons [C-10 (C carbon [C-2 ( (C 163.three), C-9 (C 163.two)], 3 aromatic 42.eight)], [C-6 quaternary carbons [C-10 (C 109.0), C-5C 75.three)], a single methylene carbon [C-3 (Ccarbons 3(C 108.four), C-7 (C 139.0), C-8109.0), C-5 (C 163.3), C-9 (carbon [C-2 (C 21.0)] (Figures S7 and S8). C 108.four), C-7 (C 139.0), (C 109.7)], and a single methyl C 163.two)], 3 aromatic carbons [C-6 ( The LR-ESI-MS information of C2 showed the peak ofmethyl carbon [C-2 (C 21.0)] (Figures S7 and S8). The LR-ESIC-8 (C 109.7)], and a single m/z 179.2 [M+H]+ (Figure S9). Compound C2 was identified as 5-hydroxy-2-methyl-chroman-4-oneof.
