Ion was essentially the most dynamic activity in the subtropical marine mangrove ecosystem. TP was the dominant environmental factor that drove the sulfur cycle and microbial communities. Sulfur bacteria, especially Desulfobacterales, are the primary executor of sulfide biotransformation. The concentrations of AS, iron, TOC, and TN in mangrove soils have been substantially larger than those in non-mangrove soils. These environmental things in mangrove soils enhanced the metabolism of sulfate reduction. Desulfobacterales and Chromatiales have been found to be responsible for the dissimilatory sulfate reduction. Additionally, Chromatiales had been mostWater 2021, 13,13 ofsensitive to environmental factors. The cysteine synthase could contribute to biotransformation of sulfide. Mangrove sediment microbiomes assimilated sulfide into L-cysteine to mitigate sulfide pollution. This study supplied a theoretical basis for the sulfur-cycle mechanism in subtropical mangrove wetland ecosystems.Supplementary Materials: The following are accessible on line at https://www.mdpi.com/article/10 .3390/w13213053/s1, Figure S1: The 20 dominant dissimilatory sulfate-reduction genes taxonomy order level are shown with their relative abundances; Figure S2: Sediment properties. (A) TOC (mg/kg), TN (mg/kg), and TP (mg/kg). (B) Contents (mg/kg) of AS and sulfide. (C) pH, salinity, and ORP. (D) Contents (mg/g) of NH4 + , NO3 – , and Fe; Figure S3: Taxonomic classification of important functional genes retrieved from the samples. (A) The key genes enriched in the MS, (B) The important genes enriched in the NMS; Figure S4: Taxonomic classification of key functional genes retrieved in the samples. (A) The crucial genes enriched in the RS, (B) The crucial genes enriched inside the NRS; Figure S5: Taxonomic classification of key functional genes retrieved in the samples. (A) cysK, (B) cysE, (C) sqr; Table S1: Functional gene abundances; Table S2: Impact of sediment properties on dissimilating sulfate-reduction gene households; Table S3: Sediment properties in mangrove samples and non-mangrove samples; Table S4: Sediment properties in rhizosphere samples and non-rhizosphere samples; Table S5: Partial Mantel test to evaluate the relative value of sediments variables in PYD-106 manufacturer determining microbial communities and the distribution of sulfur genes in subtropical mangrove ecosystem. Author Contributions: Conceptualization, C.J. and B.Y.; methodology, S.M. and J.L. (Jinhui Li); software, S.N. and M.K.; validation, B.L., J.L. (Jianping Liao) and Q.J.; formal analysis, G.S.; investigation, B.L., C.J., and B.Y.; data curation, G.S. and J.L. (Jinhui Li); writing–original draft preparation, S.M.; writing–review and editing, C.J.; visualization, S.M.; supervision, C.J.; project administration, C.J. and S.M.; funding acquisition, C.J. and B.Y. All authors have read and agreed for the published CYM51010 supplier version of the manuscript. Funding: This perform was supported by the National Organic Science Foundation of China (Grant No. 31760437), Science and Technologies Standard Sources Investigation Program of China (Grant No. 2017FY100704), the Funding Project of Chinese Central Government Guiding towards the Guangxi Neighborhood Science and Technologies Development (Grant No. GUIKEZY21195021), Organic Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Area of China (Grant No. 2019GXNSFFA245011), plus the Innovation Project of Guangxi Graduate Education (Grant No. YCSW2021064). Institutional Review Board Statement: Not applicable. Informed Co.
