Ianqian Yang1, Xiaojuan Liu1, Hui Cao2, Jianbo Xiao2, Guozheng Huang1 1 College of Life and Environmental Sciences, Shanghai Standard University, Shanghai, 201418, P. R. China; 2Institute of Chinese Medical Sciences, State Key Laboratory of High quality Investigation in Chinese Medicine, University of Macau, Avenida da Universidade, Taipa, Macau Correspondence: Jianbo Xiao [email protected] Journal of Chinese Medicine 2018, 13(Suppl 1):Chin Med 2018, 13(Suppl 1):Web page 45 ofby measuring the decrease of NADPH absorption at 340 nm, and also the dynamic absorbance was recorded for ten min at intervals of 30 s. The concentration of inhibitors represented by the half maximal inhibitory concentration (IC50) was calculated by the least-squares regression line that the concentration plotted against the residual activity. Conclusions: (1) The methylation on C5, C3, C4 of flavones remarkably weakened the inhibition; the methylation on C6, C8 of flavones enhanced the inhibition. (2) The hydroxylation on C5, C6, C7 of flavones, notably at positions 5 and six, observably enhanced the inhibition; the hydroxylation on C3 of flavones remarkably weakened the inhibition. (3) The hydrogenation of your C2=C3 double bond of flavones weakened the inhibition. (four) The glycosylation of flavonoids at distinctive positions show unique influence on their inhibitory prospective.References 1. Xiao J, Ni X, Kai G, et al. Crit Rev Meals Sci Nutr. 2015;55:16?1. two. Yeonsil L, Seonha K, Sanghoon J, et al. Biol Acid Inhibitors medchemexpress Pharmaceut Bull. 2010;33:917?1. three. Park HY, Kim HK, Jeon SH, et al. App Biol Chem. 2009;52:493?. 4. Halder N, Joshi S, Gupta SK. J Ethnopharmacol. 2003;86:113?.99 A brand new cytotoxic biflavone from Selaginella doederleinii ZhenXing Zou1, KangPing Xu2, GuoGang Zhang1, PingSheng Xu1, GuiShan Tan1 1 Xiangya Hospital of Central South University, Changsha 410008, China; two Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China Correspondence: ZhenXing Zou; GuiShan Tan Journal of Chinese Medicine 2018, 13(Suppl 1):99 Background: Selaginella doederleinii, belonging for the genus Selaginella, is broadly distributed in Amrinone manufacturer Guangxi Zhuang Autonomous Area, Guizhou and Yunnan provinces of mainland China [1]. Traditionally, the whole plant has been applied as a folk medicine to treat some types of cancers, sore throat and rheumatoid arthritis [2]. In our prior operate, some exceptional flavonoids were reported within this plant [3?]. As a part of ongoing search for novel and bioactive flavones from this genus, 75 aqueous ethanol extract from the complete herbs of Selaginella doederleinii was isolated. Components and approaches: The entire herbs of S. doederleinii were collected within the town of Wutong, Lingui district, Guangxi, China, in July 2013 and authenticated by Prof. ZhenJi Li (Xiamen University, Xiamen, China). A botanical specimen of this species (20130710) was deposited in the Xiangya School of Pharmaceutical Sciences, Central South University. Structures of 1? had been determined by extensive spectroscopic approaches like NMR and HRMS. All compounds were evaluated for their in vitro cytotoxicity against 3 human cancer cell lines A549, MCF-7, and SMMC-7721. The cytotoxicity assay was performed applying MTT (3-(four, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) method in 96-well microplates [6]. Results: A new biflavone, doederbiflavone A (1), together with four recognized ones (2?) had been isolated from S. doederleinii (Fig. 1). Compound 1 options a one of a kind dimeric skeleton with apige.
