S are problematic. The effectiveness of treatments such as interferons, colchicines, penicillamine and corticosteroids are inconsistent at best and the incidence of side-effects profound [4]. Because of the role of oxidative stress in liver cirrhosis, antioxidants have been proposed as a treatment for cirrhosis [5]. Several studies have demonstrated the protective effects of antioxidants against induced liver injury by reducing oxidative stress in cells [6,7]. A number of herbals show promising activity, including Silymarin for liver cirrhosis, glycyrrhizin for chronic viral hepatitis, and herbal combinations from China and Japan that have been proven for treatment of liver diseases [8]. Silymarin, a reference drug, is a flavonolignan from milk thistle Silybum marianum, and widely used for the treatment of hepatitis and liver cirrhosis [9].?2013 Salama et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Salama et al. BMC Complementary and Alternative Medicine 2013, 13:56 http://www.biomedcentral.com/1472-6882/13/Page 2 ofCurcuma longa is a rhizomatous perennial herb that belongs to the family Zingiberaceae, native to South Asia and is commonly known as turmeric. In Malaysia, commonly known as Kunyit, turmeric plant is a popular ingredient for preparing culinary dishes. In addition, it is used as herbal remedy due to the prevalent belief that the plant has medical properties. In folk medicine, the rhizome juice from C. longa is used in the treatment of many diseases such as anthelmintic, asthma, gonorrhea and urinary, and its essential oil is used in the treatment of carminative, stomachic and tonic [10]. In traditional medicine, several plants and herbs have been used experimentally to treat liver disorders, including liver cirrhosis, [11,12]. C. longa possesses antioxidant [13], anti-tumor [14], antimicrobial [15], anti-inflammatory [16], wound healing [17], and gastroprotective activities [18]. The previous studies have also shown that the aqueous extract of C. longa has hepatoprotective activity against carbon tetrachloride toxicity [19]. In this study, we assessed the hepatoprotective effect of the ethanolic extract of C. longa rhizomes against TAA-induced liver cirrhosis in Sprague Dawley rats.the resulting mixture was incubated in the dark for 3 min. Then, 100 L of sodium carbonate (1 g/10 mL) solution was added to the mixture, and mixed thoroughly. The final mixture was kept in the dark for 1 h and its absorbance (750 nm wavelength) was read by an ELISA reader (UV 1601 spectrophotometer, Shimadzu, Japan). All procedures were carried out in triplicate. Linear standard curves were produced by serial dilution of gallic acid (1 mg/mL DMSO) and the absorbance was read at 750 nm.Ferric reducing anti-oxidant power of CLREMethodsPreparation of CLREC. longa rhizomes were obtained from Ethno Company, Kuala Lumpur, Malaysia and identified by Mirogabalin biological activity comparison with the voucher specimen (KLU41829) deposited at the Herbarium of Rimba Ilmu, Institute of Biological Sciences, University of Malaya, Kula Lumpur, Malaysia The rhizomes were cleaned, dried, ground, weighed, and homogenized in 95 ethanol at a ratio of 1:10 of plant to ethanol and left to soak for 3 days at 25 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26100631 with occasional shaking and s.
