Tic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation. 1317923 Proc Natl Acad Sci U S A 104: 59655970. 7. Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Procedures 33: 213219. 8. Breunig JJ, Arellano JI, Macklis JD, Rakic P Every little thing that glitters is not gold: a critical assessment of postnatal neural precursor analyses. Cell Stem Cell 1: 612627. 9. Chabes A, Stillman B Constitutively higher dNTP concentration inhibits cell cycle progression as well as the DNA damage checkpoint in yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 104: 11831188. ten. Kumar D, Viberg J, Nilsson AK, Chabes A Hugely mutagenic and severely imbalanced dNTP pools can escape detection by the S-phase checkpoint. Nucleic Acids Res 38: 39753983. 11. Kumar D, Abdulovic AL, Viberg J, Nilsson AK, Kunkel TA, et al. Mechanisms of mutagenesis in vivo as a consequence of imbalanced dNTP pools. Nucleic Acids Res 39: 13601371. 12. Lixisenatide Harper JV Synchronization of cell populations in G1/S and G2/M phases of the cell cycle. Procedures Mol Biol 296: 157166. 13. Hakansson P, Dahl L, Chilkova O, Domkin V, Thelander L The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding for the significant Cdc22 subunit. J Biol Chem 281: 17781783. 14. Lasken RS, Goodman MF The biochemical basis of 5-bromouracilinduced mutagenesis. Heteroduplex base mispairs involving bromouracil in G 6 C-A six T and also a 6 T-G six C mutational pathways. J Biol Chem 259: 11491 11495. 15. Hua H, Kearsey SE Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-29-deoxyuridine. Nucleic Acids Res 39: e60. 16. Sabatinos SA, Mastro TL, Green MD, Forsburg SL A Mammalian-Like DNA Harm Response of Fission Yeast to Nucleoside Analogues. Genetics 193: 143157. 17. Moreno S, Klar A, Nurse P Molecular genetic evaluation of fission yeast Schizosaccharomyces pombe. Techniques Enzymol 194: 795823. 18. Knutsen JH, Rein ID, Rothe C, Stokke T, Grallert B, et al. Cell-cycle evaluation of fission yeast cells by flow cytometry. PLoS 1 6: e17175. 8 Cell-Cycle Analyses Applying Thymidine Analogues 19. Nilssen EA, Synnes M, Kleckner N, Grallert B, Boye E Intra-G1 arrest in response to UV irradiation in fission yeast. Proc Natl Acad Sci U S A 100: 1075810763. 20. Patel PK, Arcangioli B, Baker SP, Bensimon A, Rhind N DNA replication origins fire stochastically in fission yeast. Mol Biol Cell 17: 308316. 21. Tvegard T, Soltani H, Skjolberg HC, Krohn M, Nilssen EA, et al. A novel checkpoint mechanism regulating the G1/S transition. Genes Dev 21: 649654. 22. Alvino GM, Collingwood D, Murphy JM, Delrow J, Brewer BJ, et al. Replication in hydroxyurea: it’s a matter of time. Mol Cell Biol 27: 63966406. 23. Kim SM, Huberman JA Regulation of replication timing in fission yeast. Embo J 20: 61156126. 24. Sabatinos SA, Green MD, Forsburg SL Continued DNA synthesis in replication checkpoint mutants results in fork collapse. Mol Cell Biol 32: 4986 4997. 25. Yonemasu R, McCready SJ, get CAL-120 Murray JM, Osman F, Takao M, et al. Characterization of the alternative excision repair pathway of UV-damaged DNA in Schizosaccharomyces pombe. Nucleic Acids Res 25: 15531558. 26. McCready SJ, Osman F, Yasui A Repair of UV damage inside the fission yeast Schizosaccharomyces pombe. Mutat Res 451: 197210. 27. Boe CA, Krohn M, Rodland GE, Capiaghi C, Maillard O, et al. Induction of a G1-S checkpoint in fissio.Tic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation. 1317923 Proc Natl Acad Sci U S A 104: 59655970. 7. Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Strategies 33: 213219. 8. Breunig JJ, Arellano JI, Macklis JD, Rakic P Every thing that glitters isn’t gold: a crucial review of postnatal neural precursor analyses. Cell Stem Cell 1: 612627. 9. Chabes A, Stillman B Constitutively higher dNTP concentration inhibits cell cycle progression and the DNA harm checkpoint in yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 104: 11831188. 10. Kumar D, Viberg J, Nilsson AK, Chabes A Hugely mutagenic and severely imbalanced dNTP pools can escape detection by the S-phase checkpoint. Nucleic Acids Res 38: 39753983. 11. Kumar D, Abdulovic AL, Viberg J, Nilsson AK, Kunkel TA, et al. Mechanisms of mutagenesis in vivo due to imbalanced dNTP pools. Nucleic Acids Res 39: 13601371. 12. Harper JV Synchronization of cell populations in G1/S and G2/M phases from the cell cycle. Techniques Mol Biol 296: 157166. 13. Hakansson P, Dahl L, Chilkova O, Domkin V, Thelander L The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the massive Cdc22 subunit. J Biol Chem 281: 17781783. 14. Lasken RS, Goodman MF The biochemical basis of 5-bromouracilinduced mutagenesis. Heteroduplex base mispairs involving bromouracil in G 6 C-A six T as well as a 6 T-G 6 C mutational pathways. J Biol Chem 259: 11491 11495. 15. Hua H, Kearsey SE Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-29-deoxyuridine. Nucleic Acids Res 39: e60. 16. Sabatinos SA, Mastro TL, Green MD, Forsburg SL A Mammalian-Like DNA Harm Response of Fission Yeast to Nucleoside Analogues. Genetics 193: 143157. 17. Moreno S, Klar A, Nurse P Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Techniques Enzymol 194: 795823. 18. Knutsen JH, Rein ID, Rothe C, Stokke T, Grallert B, et al. Cell-cycle analysis of fission yeast cells by flow cytometry. PLoS A single 6: e17175. 8 Cell-Cycle Analyses Using Thymidine Analogues 19. Nilssen EA, Synnes M, Kleckner N, Grallert B, Boye E Intra-G1 arrest in response to UV irradiation in fission yeast. Proc Natl Acad Sci U S A one hundred: 1075810763. 20. Patel PK, Arcangioli B, Baker SP, Bensimon A, Rhind N DNA replication origins fire stochastically in fission yeast. Mol Biol Cell 17: 308316. 21. Tvegard T, Soltani H, Skjolberg HC, Krohn M, Nilssen EA, et al. A novel checkpoint mechanism regulating the G1/S transition. Genes Dev 21: 649654. 22. Alvino GM, Collingwood D, Murphy JM, Delrow J, Brewer BJ, et al. Replication in hydroxyurea: it’s a matter of time. Mol Cell Biol 27: 63966406. 23. Kim SM, Huberman JA Regulation of replication timing in fission yeast. Embo J 20: 61156126. 24. Sabatinos SA, Green MD, Forsburg SL Continued DNA synthesis in replication checkpoint mutants results in fork collapse. Mol Cell Biol 32: 4986 4997. 25. Yonemasu R, McCready SJ, Murray JM, Osman F, Takao M, et al. Characterization of your alternative excision repair pathway of UV-damaged DNA in Schizosaccharomyces pombe. Nucleic Acids Res 25: 15531558. 26. McCready SJ, Osman F, Yasui A Repair of UV harm in the fission yeast Schizosaccharomyces pombe. Mutat Res 451: 197210. 27. Boe CA, Krohn M, Rodland GE, Capiaghi C, Maillard O, et al. Induction of a G1-S checkpoint in fissio.