Amidi C, Maaskola J, Einspanier R, et al. Discovering microRNAs from deep sequencing data employing miRDeep. Nature biotechnology 26: 407415. 20. Livak KJ, Schmittgen TD Analysis of relative gene expression data employing real-Time quantitative PCR plus the 22ggCT approach. Strategies 25: 402408. 21. Allen E, Xie Z, Gustafson AM, Carrington JC microRNA-directed phasing for the duration of trans-acting siRNA biogenesis in plants. Cell 121: 207221. 22. Schwab R, Palatnik JF, Riester M, Schommer C, Schmid M, et al. Certain effects of microRNAs around the plant transcriptome. Dev Cell 8: 517527. 23. Quevillon E, Silventoinen V, Pillai S InterProScan: protein domains identifier. Nucleic Acids Res 33: 116120. eight microRNAs Laying and Broody Geese 24. Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, et al. B1ast2GO: a universal tool for annotation, visualization and analysis in functional genomics investigation. Bioinformatics 21: 36743676. 25. Carbon S, Ireland A, Mungall CJ, Shu S, Marshall B, et al. AmiGO: on line access to ontology and annotation information. Bioinformatics 25: 288289. 26. Smoot ME, Ono K, Ruscheinski J, Wang PL, Ideker T Cytoscape 2.eight: new characteristics for data integration and network visualization. Bioinformatics 27: 431432. 27. Bindea G, Mlecnik B, Hackl H, Charoentong P, Tosolini M, et al. C1ueG0: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks. Bioinformatics 25: 10911093. 28. Kanehisa M, Goto S, Sato Y, Furumichi M, Tanabe M KEGG for integration and interpretation of large-scale molecular datasets. Nucleic Acids Res 40: 109114. 29. Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, et al. A ASP015K price mammalian microRNA expression atlas primarily based on compact RNA library sequencing. Cell, 129: 14011414. 30. Ro S, Song R, Park C, Zheng H, Sanders KM, et al. Cloning and expression profiling of modest RNAs expressed within the mouse ovary. RNA, 13: 23662380. 31. Mishima T, Takizawa T, Luo SS, Ishibashi O, Kawahigashi Y, et al. MicroRNA cloning analysis reveals sex differences in miRNA expression profiles involving adult mouse testis and ovary. Reproduction, 136: 811822. 32. Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, et al. MicroRNA transcriptome inside the newborn mouse ovaries determined by massive parallel sequencing. Mol Hum Reprod, 16: 463471. 33. Li M, Liu Y,Wang T, Guan J, Luo Z, et al. Repertoire of porcine microRNAs in adult ovary and testis by deep sequencing. Int J Biol 18325633 Sci, 7: 1045 1055. 34. Hossain MM, Ghanem N, Hoelker M, Rings F, Phatsara C, et al. Identification and characterization of miRNAs expressed inside the bovine ovary. BMC Genomics, 10: 443. 35. Tripurani SK, Xiao C, Salem M, Yao J Cloning and evaluation of fetal ovary microRNAs in cattle.Anim Reprod Sci, 120: 1622. 36. Miles JR, McDaneld TG, Wiedmann RT, Cushman RA, Echternkamp SE, et al. MicroRNA expression profile in bovine cumulusoocyte complexes: feasible function of let-7 and miR-106a inside the improvement of bovine oocytes. Anim Reprod Sci, 130: 1626. 37. McBride D, Carre W, Sontakke S, Hogg CO, Law AS, et al. Identification of miRNAs CASIN site connected with the follicularluteal transition within the ruminant ovary. Reproduction, 144: 221233. 38. Zhang XD, Ling YH, Zhang YH, Li YS, Liu Ya, et al. MicroRNAs in Ovaries of Goats Identified by Solexa Sequencing. Scientia Agricultura Sinica, 46: 146153. 39. Wang XG, Yu JF, Zhang Y, Gong DQ, Gu ZL Identification and characterization of microRNAs from chicken adipose tissue and skeletal muscle. Poult Sci, 91:13949. 40. Yao J, Wang Y, Wang W,.Amidi C, Maaskola J, Einspanier R, et al. Discovering microRNAs from deep sequencing information using miRDeep. Nature biotechnology 26: 407415. 20. Livak KJ, Schmittgen TD Evaluation of relative gene expression data using real-Time quantitative PCR plus the 22ggCT system. Methods 25: 402408. 21. Allen E, Xie Z, Gustafson AM, Carrington JC microRNA-directed phasing through trans-acting siRNA biogenesis in plants. Cell 121: 207221. 22. Schwab R, Palatnik JF, Riester M, Schommer C, Schmid M, et al. Distinct effects of microRNAs around the plant transcriptome. Dev Cell 8: 517527. 23. Quevillon E, Silventoinen V, Pillai S InterProScan: protein domains identifier. Nucleic Acids Res 33: 116120. eight microRNAs Laying and Broody Geese 24. Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, et al. B1ast2GO: a universal tool for annotation, visualization and evaluation in functional genomics analysis. Bioinformatics 21: 36743676. 25. Carbon S, Ireland A, Mungall CJ, Shu S, Marshall B, et al. AmiGO: on the internet access to ontology and annotation information. Bioinformatics 25: 288289. 26. Smoot ME, Ono K, Ruscheinski J, Wang PL, Ideker T Cytoscape 2.eight: new capabilities for data integration and network visualization. Bioinformatics 27: 431432. 27. Bindea G, Mlecnik B, Hackl H, Charoentong P, Tosolini M, et al. C1ueG0: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks. Bioinformatics 25: 10911093. 28. Kanehisa M, Goto S, Sato Y, Furumichi M, Tanabe M KEGG for integration and interpretation of large-scale molecular datasets. Nucleic Acids Res 40: 109114. 29. Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, et al. A mammalian microRNA expression atlas primarily based on modest RNA library sequencing. Cell, 129: 14011414. 30. Ro S, Song R, Park C, Zheng H, Sanders KM, et al. Cloning and expression profiling of modest RNAs expressed in the mouse ovary. RNA, 13: 23662380. 31. Mishima T, Takizawa T, Luo SS, Ishibashi O, Kawahigashi Y, et al. MicroRNA cloning evaluation reveals sex variations in miRNA expression profiles amongst adult mouse testis and ovary. Reproduction, 136: 811822. 32. Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, et al. MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing. Mol Hum Reprod, 16: 463471. 33. Li M, Liu Y,Wang T, Guan J, Luo Z, et al. Repertoire of porcine microRNAs in adult ovary and testis by deep sequencing. Int J Biol 18325633 Sci, 7: 1045 1055. 34. Hossain MM, Ghanem N, Hoelker M, Rings F, Phatsara C, et al. Identification and characterization of miRNAs expressed within the bovine ovary. BMC Genomics, ten: 443. 35. Tripurani SK, Xiao C, Salem M, Yao J Cloning and analysis of fetal ovary microRNAs in cattle.Anim Reprod Sci, 120: 1622. 36. Miles JR, McDaneld TG, Wiedmann RT, Cushman RA, Echternkamp SE, et al. MicroRNA expression profile in bovine cumulusoocyte complexes: achievable function of let-7 and miR-106a within the improvement of bovine oocytes. Anim Reprod Sci, 130: 1626. 37. McBride D, Carre W, Sontakke S, Hogg CO, Law AS, et al. Identification of miRNAs linked to the follicularluteal transition within the ruminant ovary. Reproduction, 144: 221233. 38. Zhang XD, Ling YH, Zhang YH, Li YS, Liu Ya, et al. MicroRNAs in Ovaries of Goats Identified by Solexa Sequencing. Scientia Agricultura Sinica, 46: 146153. 39. Wang XG, Yu JF, Zhang Y, Gong DQ, Gu ZL Identification and characterization of microRNAs from chicken adipose tissue and skeletal muscle. Poult Sci, 91:13949. 40. Yao J, Wang Y, Wang W,.